human mouse rat porcine canine tgf beta 1 elisa kit  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Development and validation of a potency assay matrix for optimized and consistent manufacture of clinical mesenchymal stem/stromal cells

doi: 10.3389/fimmu.2026.1725191

Figure Lengend Snippet: MSC-derived EVs drive Treg polarization. (A) Representative flow cytometry plots and quantification of FoxP3 and CD25 expression in CD4 + T cells treated with different numbers of EVs or without EVs (Ctrl). Data are means ± SD. n = 4 technical replicates using one PBMC donors. (B) IL-10 levels in the supernatant of T cells incubating with a range of EV numbers or without EVs. Data are means ± SD. n = 3 technical replicates using one PBMC donors. (C) Replication index ratio of FoxP3-positive vs. FoxP3-negative CD4 + T cells treated with different numbers of EVs or without EVs. Data are means ± SD. n = 3 technical replicates using one PBMC donors. (D) Representative imaging flow cytometry images of CD4 + and CD8 + T cells incubated with CBO-labeled EVs for 24hrs. (E) ELISA TGFβ1 levels on EVs (4 x 10 11 EVs used for ELISA) from MSC-CM derived from three different MSC donors. Data are means ± SD. n = 3 technical replicates using three independent EVs preparations per MSC donor. (F) Frequency of FoxP3 + cells among CD4 + T cells treated with 4 x 10 11 EVs for 3 days in combination with 5µM SB431542 or 1µM A83–01 or vehicle control (DMSO) or incubated without EVs (Ctrl). Data are means ± SD. n = 3 technical replicates using one PBMC donors. (G) Frequency of FoxP3 + cells among CD4 + T cells treated with or without 4 x 10 11 EVs for 3 days ± 5µg/mL anti-TGFβ1 antibody or isotype control. Data are means ± SD. n ≥ 3 technical replicates using ≥ 3 different PMBC donors. Statistical significance was determined using one-way ANOVA with Tukey’s HSD test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

Article Snippet: TGFβ1 concentration was evaluated using the TGFβ1 ELISA kit (R&D Systems, DB100C) following manufacturer’s instructions.

Techniques: Derivative Assay, Flow Cytometry, Expressing, Imaging, Incubation, Labeling, Enzyme-linked Immunosorbent Assay, Control

Journal: Materials Today Bio

Article Title: Interferon-stimulated gene 15 small interfering RNA-loaded polarized mesoporous silica nanocarriers remodel the immune microenvironment to ameliorate post-myocardial infarction heart failure

doi: 10.1016/j.mtbio.2025.102631

Figure Lengend Snippet: pMSNC@ISG15 siRNA alleviates cardiomyocyte injury. Note: (A) Schematic diagram of the experimental workflow for simulating the immune microenvironment of Post-MI HF via PRCM and BMDM co-culture; (B–C) Western blot analysis of ISG15 protein expression in a CoCl 2 -induced hypoxia model; (D) RT-qPCR analysis of ISG15 mRNA levels in the same model; (E) ELISA quantification of pro-inflammatory cytokines (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-10, TGF-β) in co-culture supernatants; (F) Assessment of CK and CK-MB release; (G) Measurement of LDH release; (H) Detection of cTnI levels; (I) CCK-8 assay evaluating cell viability. All cell-based experiments were performed in triplicate. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 indicate statistical significance between groups.

Article Snippet: Inflammatory cytokines (IL-10, TGF-β, TNF-α, and IL-1β) were quantified using ELISA kits from R&D Systems (catalog numbers R1000, DB100C, RTA00-1, and RLB00-1, respectively).

Techniques: Co-Culture Assay, Western Blot, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, CCK-8 Assay

Journal: Materials Today Bio

Article Title: Interferon-stimulated gene 15 small interfering RNA-loaded polarized mesoporous silica nanocarriers remodel the immune microenvironment to ameliorate post-myocardial infarction heart failure

doi: 10.1016/j.mtbio.2025.102631

Figure Lengend Snippet: Modulation of macrophage phenotype by pMSNC@ISG15 siRNA. Note: (A) Live/Dead fluorescence staining showing the viability of PRCMs and BMDMs treated with pMSNC@ISG15 siRNA at various time points (bar = 25, 50 μm); (B) Experimental workflow illustrating the regulatory effect of ISG15 siRNA-loaded pMSNCs on M2 macrophage activation; (C–D) Flow cytometry analysis of pro-inflammatory (M1) and anti-inflammatory (M2) macrophage subsets; (E–F) IF staining of macrophage markers iNOS and Arg-1 (bar = 12, 25 μm); (G–J) ELISA quantification of anti-inflammatory cytokines (IL-10, TGF-β) and pro-inflammatory cytokines (TNF-α, IL-1β). All cell-based experiments were performed in triplicate. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 indicates statistical significance between groups.

Article Snippet: Inflammatory cytokines (IL-10, TGF-β, TNF-α, and IL-1β) were quantified using ELISA kits from R&D Systems (catalog numbers R1000, DB100C, RTA00-1, and RLB00-1, respectively).

Techniques: Fluorescence, Staining, Activation Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: Interferon-stimulated gene 15 small interfering RNA-loaded polarized mesoporous silica nanocarriers remodel the immune microenvironment to ameliorate post-myocardial infarction heart failure

doi: 10.1016/j.mtbio.2025.102631

Figure Lengend Snippet: Therapeutic effect of pMSNC@ISG15 siRNA in preventing Post-MI HF. Note: (A) Schematic diagram illustrating the experimental procedure, in which drug-loaded functionalized silica nanoparticles deliver ISG15 siRNA to suppress immune cell activation in the cardiac microenvironment and reverse post-MI HF; (B–D) Echocardiographic evaluation of cardiac function across different treatment groups; (E) ECG analysis of cardiac function in each group; (F) ELISA quantification of anti-inflammatory cytokines (IL-10, TGF-β) and pro-inflammatory cytokines (TNF-α, IL-1β) in treated rats; (G) Measurement of LDH release to assess myocardial injury across groups; (H) Detection of cTnI levels as a marker of myocardial damage; (I) DHE fluorescent probe assay to measure ROS levels in myocardial tissue from each treatment group. Each group included 10 animals. ∗∗ p < 0.01, ∗∗∗ p < 0.001 indicate statistical significance between groups.

Article Snippet: Inflammatory cytokines (IL-10, TGF-β, TNF-α, and IL-1β) were quantified using ELISA kits from R&D Systems (catalog numbers R1000, DB100C, RTA00-1, and RLB00-1, respectively).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Marker